Cultivation Technology Of Shiitake Mushroom (Lentinula Edodes)

Cultivation technology of Shiitake mushroom (Lentinula edodes)

The cultivation technology of the edible and medicinal mushroom Lentinula edodes commonly called as Shiitake mushroom has been standardized. The flow chart of the technology is as follows.

Substrate preparation

(Mixture of hard wood sawdust, wood chips, Rice bran and calcium sulphate)

Fill one Kg wet substrate (65% moisture) to Polypropylene bags, plug with non-absorbent cotton 

Sterilize the substrate (121°C, 15 lb pressure for 90-120 minutes in autoclave)

Cool the bags to room temperature

Spawning (Aseptically, @ 5% of wet substrate)

Spawn running (22-26°C, 60-70 days)


Initial spawn running


Mycelial coat formation


Blister formation


Open bags for browning

Cold water shock treatment (10-15°C water, 6-8 hours)

Fructification (22-26°C, 80-85% R.H, Light, cross ventilation)


Pinheads     


Ready to harvest mushrooms

Sold as fresh or dry mushroom

Services offered by the mushroom lab, IIHR

Spawn of commercial varieties of Oyster mushroom (Pleurotus species), Milky mushroom (Calocybe indica), Shirotamogitake (Hypsizygous ulmarius) and Shiitake (Lentinula edodes) can be procured in person on prior order basis.

Regular training courses are offered on Mushroom seed production and Mushroom cultivation technology separately.

Literature – Mushroom cultivation Bulletin, folders on mushroom cultivation.

Further details: Director, IIHR, Hessaraghatta lake post, Hessaraghatta, Bangalore-560089, E-mail:director@iihr.ernet.in