Cultivation Technology Of Milky Mushroom

The cultivation technology of Calocybe spp. commonly called as Milky mushroom has been standardized on locally available substrates. This is the first Indian mushroom variety to be commercialized by IIHR. This variety is a tropical species and has excellent shelf life. The flow chart of the technology is as follows.

Substrate preparation

(Paddy or wheat straw) Chopped in small (1-2cm pieces), soaked in water for 2-3 hours

Substrate can be pasteurized by hot water (80°C) for 2hours (no prior soaking required in this case), suitable for small scale production OR steam pasteurization at 80°C for 2 hours (in autoclave or special steam chambers), suitable for semi-commercial production OR sterilized in an autoclave (after filling in PP bags, plugged with cotton) at 121°C, 15 lb pressure for 15 minutes (best method for commercial production)

Hot water pasteurization

steam pasteurization


Fill one Kg pasteurized substrate (65% moisture) in Polypropylene bags, plug with non-absorbent cotton

Spawning (Aseptically, @ 5% of wet substrate) while filling in bags in pasteurization method OR top spawning in sterilized method

Spawning in pasteurized bags

 Top spawning in sterilized bags

Spawn running in dark room (30-38°C, 25-30 days)

Casing with a mixture of red soil or red soil+ Decomposed coir compost (1:1), pH 8.0

Shift to cropping room after casing, 30-38°C, humidity 80-85%, light, ventilation

Pinhead initiation (7-10 days after casing)

 Harvesting (3-4 days after pinhead initiation, 30-38°C, humidity 80-85%, light, ventilation)

Sold as fresh, dry mushroom or as mushroom products

Services offered by the mushroom lab, IIHR

Spawn of commercial varieties of Oyster mushroom (Pleurotus species), Milky mushroom (Calocybe indica), Shirotamogitake (Hypsizygous ulmarius) and Shiitake (Lentinula edodes) can be procured in person on prior order basis.

Regular training courses are offered on Mushroom seed production and Mushroom cultivation technology separately. 

Literature – Mushroom cultivation Bulletin, folders on mushroom cultivation.

Further details: Director, IIHR, Hessaraghatta lake post, Hessaraghatta, Bangalore-560089,